Isolation, screening and characterization ofbacillus sp. for whey based polyhydroxyalkanoate (pha) production

Abstract

Prachi Sahu, Namrata Adivi, Ritika Mahajan, Manusha N, Aarthi Nanda Kumar, Harshitha D.M, Mohammad Shahid, Srinivas G and Srividya Shivakumar*

Whey is an excellent source of carbon for bacteria that produce Polyhydroxyalkanoates (PHA). There are no research employing raw whey, which has a very small amount of lactose, despite the fact that the majority of studies have utilized Whey, which includes large quantities of lactose as ingredient. Based on Sudan Black B colony staining, the following 30 bacterial strains were identified and chosen for further screening: S4, S8, S9, C10, C11, C12, C13, S15, S33, S35, and S37. The ideal circumstances for PHA synthesis will next be looked at, and the most effective isolate will be found using molecular characterization. 30 bacterial isolates were subjected to a two-step throughput screening procedure utilizing the lipophilic stain (Sudan black B) or fluorescence stain (Nile red) methods. Sudan Black and Nile Red, two separate indicator stains, will be used to examine bacterial isolates for PHA generation before the most productive isolate is found. On an increase in PHA production, which will be measured by converting PHA to crotonic acid, the research of variables influencing PHA production and stressful situations will alternatively rely. The biopolymer will be extracted using a variety of solvents. The current work offers a first exploration into finding and isolating a powerful Bacillus species that may produce PHA from dairy waste (whey). Helps handle dairy waste as solid waste, which reduces pollution and speeds up the recycling process.