Effect of Ethyl Methyl Sulfonate on Cyto-Morphological variations in Corchorus capsularis L.

Abstract

Jyoti Yadav, Girjesh Kumar, Moni Mishra* and Priyanka Ojha

Dry seeds of Corchorus capsularis L. were treated with three doses of Ethyl methyl sulfonate viz. 0.1%, 0.3% and 0.5%. In a laboratory test, AMI% was decreased and TAB% was increased with increases in the concentration of mutagen. Similarly, germination percentage and survival were also negatively affected by an increase in concentration during the field study. In this experiment, four types of chlorophyll mutation viz. maculata, chlorina, xantha, and aurea were observed xentha and aurea were observed in most of the treated sets, whereas, . meculata and chlorina observed only in lower doses viz. 0.1% and 0.3%. Based on the findings during experiment, a negative correlation was obtained between AMI, root length, shoot length, chlorophyll mutants, and concentration of EMS. Results show an increment in chromosomal anomaly at higher concentrations and a wide range of abnormalities were recorded i.e., stickiness, scattering, unorientation, bridges, etc. The application of EMS was affected survival considerably than the germination%.