Design of a Bioassay for ecopharmacovigilance studies in Phaseolus vulgaris.

Abstract

Mex-Alvarez Rafael Manuel de Jesus, Guillen-Morales María Magali, Garma-Quen Patricia Margarita, Yanez-Nava David, Chan-Martínez Roger Enrique, Liubov Leliavska Fonseca-Calderón William Benjamin

Phaseolus vulgaris is a plant species recommended and used for Eco toxicity and phytotoxicity bioassays due to its sensitivity and the production of secondary metabolites that can act as exposure indicators. The objective of this research work was to design a bioassay for the cultivation of P. vulgaris in agar-agar to facilitate its exposure by homogeneous diffusion of chemical substances in the agar and the integrity of the plant root when harvested. The growth of P. vulgaris was evaluated at different concentrations of agar and at different days of culture and morphometric measurements of the plants and determination of total chlorophyll, total carotenoids, total polyphenols and antioxidant activity of the leaves of the cultivated plants were carried out. It was found that the best conditions due to growth and production of secondary metabolites were growing P. vulgaris in 0.5% agar for 7 days. This bioassay is available for use in Eco pharmacovigilance studies of drugs as environmental contaminants.